Plasma/Serum Exosome Purification and RNA Isolation Maxi Kit
供应商
Norgen Biotek
产品货号
NGB-58600
产品报价
¥询价/15Preps
产品说明书
购买方式
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产品新闻
背景资料
无内容
产品描述
Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Maxi Kit constitutes an all-in-one system for the purification of exosomes and the sequential isolation of exosomal RNA and free-circulating RNA from different plasma/serum sample volumes ranging from 4 mL and up to 10 mL. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or any protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
产品特点
Purification and enrichment of intact plasma/serum exosomes for functional studies; Bind and elute all RNA irrespective of size or GC content, without bias; Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA; Versatile plams/serum input volume (4 mL - 10 mL); No phenol extractions, Proteinase K treatment, nor carrier RNA required; No time-consuming ultracentrifugation, filtration nor special syringes required; No precipitation reagents nor overnight incubation required; Compatible with plasma/serum from any species; Pure exosomes are purified and are free-from any other RNA-binding proteins.